Repeated cycles of amplification cause degradation with the probe, leading to amplified fluorescence. TaqMan detection is, for that reason, much more distinct than dye‐dependent detection, due to the fact a rise in fluorescence happens only upon binding in the probe to a particular sequence and subsequent de‐quenching, although no sign https://www.facebook.com/NewTechFind/posts/pfbid02RncFQX8CkjAPpZAaHcChBuCRzeG2YdMLeWZbJ9C4o1fi9ZzNf6Lyqa9d8ohEWRjsl